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Serum concentrations and expressions of serum amyloid A and leptin in adipose tissue are interrelated: the Genobin Study

¹ Department of Clinical Nutrition and Food and Health Research Center, University of Kuopio, PO Box 1627, FIN-70211 Kuopio, Finland² Institute of Clinical Medicine, Internal Medicine, Kuopio University Hospital, PO Box 1777, FIN-70211 Kuopio, Finland³ Department of Physiology and ⁴ Kuopio Research Institute of Exercise Medicine, University of Kuopio, PO Box 1627, FIN-70211 Kuopio, Finland

(Correspondence should be addressed to T Lappalainen; Email: tiina.lappalainen{at}uku.fi)

Objective: Serum amyloid A (SAA) is a novel link between increased adipose tissue mass and low-grade inflammation in obesity. Little is known about the factors regulating its serum concentration and mRNA levels. We investigated the association between SAA and leptin in obese and normal weight subjects and analyzed the effect of weight reduction on serum SAA concentration and gene expression in adipose tissue of the obese subjects.

Methods: Seventy-five obese subjects (60±7 years, body mass index (BMI) 32.9±2.8 kg/m², mean±S.D.) with impaired fasting plasma glucose or impaired glucose tolerance and other features of metabolic syndrome, and 11 normal weight control subjects (48±9 years, BMI 23.7±1.9 kg/m²) were studied at the baseline. Twenty-eight obese subjects underwent a 12-week intensive weight reduction program followed by 5 months of weight maintenance. Blood samples and abdominal s.c. adipose tissue biopsies were taken at the baseline and after the follow-up. Gene expression was studied using real-time quantitative PCR.

Results: The gene expressions in women and serum concentrations of leptin and SAA were interrelated independently of body fat mass in the obese subjects (r=0.54, P=0.001; r=0.24, P=0.039 respectively). In multiple linear regression analyses, leptin mRNA explained 38% of the variance in SAA mRNA (P=0.002) in the obese women. Weight loss of at least 5% increased SAA mRNA expression by 48 and 36% in men and women, but serum SAA concentrations did not change.

Conclusions: The association between SAA and leptin suggests an interaction between these two adipokines, which may have implications in inflammatory processes related to obesity and the metabolic syndrome.