Eur J Endocrinol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

DOI: 10.1530/EJE-07-0017
European Journal of Endocrinology, Vol 156, Issue 6, 695-702
Copyright © 2007 by European Society of Endocrinology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stoykov, I
Right arrow Articles by Bakker, O
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stoykov, I
Right arrow Articles by Bakker, O
EXPERIMENTAL STUDY

Effect of amiodarone and dronedarone administration in rats on thyroid hormone-dependent gene expression in different cardiac components

I Stoykov1,2, H C van Beeren1, A F M Moorman2, V M Christoffels2, W M Wiersinga1 and O Bakker1

1 Department of Endocrinology and Metabolism and 2 Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands

(Correspondence should be addressed to O Bakker; Email: o.bakker{at}amc.uva.nl)

Objective: In view of their different actions on thyroid hormone receptor (TR) isoforms we set out to investigate whether amiodarone (AM) and dronedarone (Dron) have different and/or component-specific effects on cardiac gene expression.

Design: Rats were treated with AM or Dron and the expression of TR{alpha} 1, TR{alpha} 2, TRß 1 and several tri-iodothyronine (T3)-regulated genes was studied in different parts of the heart, namely the right atrium (RA), left ventricular wall (LVW) and apex.

Methods: Rats were treated for 14 days with 100 mg/kg body weight AM or Dron. The expression of TR{alpha} 1, TR{alpha} 2, TRß 1 and T3-regulated genes was studied using real-time PCR and non-radioactive in situ hybridisation.

Results: AM and Dron affected TR expression in the RA similarly by decreasing TR{alpha} 1 and ß 1 expression by about 50%. In the LVW, AM and Dron decreased TRß 1 and, interestingly, AM increased TR{alpha} 1. In the apex, AM also increased TR{alpha} 2. The changes seen in T3-dependent gene expression are reminiscent of foetal reprogramming.

Conclusion: Taken together, our results indicate that AM and Dron have similar effects on the expression of TR isoforms in the RA, which could partly contribute to their ability to decrease heart rate. On the other hand, the more profound effect of AM appears on TR- and T3-dependent gene expression in the left ventricle suggests foetal reprogramming.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 European Society of Endocrinology.