Eur J Endocrinol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

DOI: 10.1530/eje.0.1390454
European Journal of Endocrinology, Vol 139, Issue 4, 454-460
Copyright © 1998 by European Society of Endocrinology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, S
Right arrow Articles by Seino, Y
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, S
Right arrow Articles by Seino, Y
Articles

Local action of exogenous growth hormone and insulin-like growth factor-I on dihydroxyvitamin D production in LLC-PK1 cells

S Wei, H Tanaka, and Y Seino

Department of Pediatrics, Okayama University Medical School, Japan.

Previous in vivo studies have shown that growth hormone (GH) affects vitamin D and mineral metabolism. Insulin-like growth factor-I (IGF-I) was recently reported to be a regulator of renal 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) production, suggesting that it mediates the effects of GH on vitamin D metabolism. However, there is no direct evidence to support this. The present study was designed to investigate the in vitro effects of GH and IGF-I on the renal production of 1,25-(OH)2D3 and 24,25-dihydroxyvitamin D3 (24,25-(OH)2D3) in a pig kidney cell line, LLC-PK1. Confluent cells were preincubated in serum-free medium with hormone (GH or IGF-I) or vehicle, and then incubated with 25-[3H]OHD3. The levels of 1,25-[3H](OH)2D3 and 24,25-[3H](OH)2D3 produced were determined after lipid extraction and HPLC purification. Production of 1,25-(OH)2D3 and 24,25-(OH)2D3 was increased after both IGF-I and GH preincubation in a dose-dependent manner. Significant increases were found after preincubation with 13 nmol/l IGF-I (1,25-(OH)2D3, 1.8-fold: 24,25-(OH)2D3, 1.5-fold)or 0.9 or 9 nmol/lGH (1,25-(OH)2D3, 1.3-fold and 1.5-fold; 24.25-(OH)2D3, 1.4-fold and 1.5-fold respectively). Furthermore, the effect of 9 nmol/l GH on 1.25-(OH)2D3 and 24,25-(OH)2D3 production was blocked in the presence of IGF-I receptor monoclonal antibody. These results confirm that IGF-I acts on renal tubules, resulting in induction of 1,25-(OH)2D3 and 24,25-(OH)2D3 production, and the findings suggest that GH stimulates 1.25-(OH)2D3 and 24,2 5-(OH)2D3 production by increasing local IGF-I production in the kidney.


This article has been cited by other articles:


Home page
 J. Nutr.Home page
P. D. Gluckman and C. S. Pinal
Regulation of Fetal Growth by the Somatotrophic Axis
J. Nutr., May 1, 2003; 133(5): 1741S - 1746.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Halhali, A. R. Tovar, N. Torres, H. Bourges, M. Garabedian, and F. Larrea
Preeclampsia Is Associated with Low Circulating Levels of Insulin-Like Growth Factor I and 1,25-Dihydroxyvitamin D in Maternal and Umbilical Cord Compartments
J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 1828 - 1833.
[Abstract] [Full Text]

Home page
 J. Nutr.Home page
M.-S. Wong, V. A. Tembe, and M. J. Favus
Insulin-Like Growth Factor-I Stimulates Renal 1,25-Dihydroxycholecalciferol Synthesis in Old Rats Fed a Low Calcium Diet
J. Nutr., May 1, 2000; 130(5): 1147 - 1152.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
A. Halhali, L. Diaz, I. Sanchez, M. Garabedian, H. Bourges, and F. Larrea
Effects of IGF-I on 1,25-dihydroxyvitamin D3 synthesis by human placenta in culture
Mol. Hum. Reprod., August 1, 1999; 5(8): 771 - 776.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 European Society of Endocrinology.