| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Dumon JC, Wantier H, Mathieu F, Mantia M, Body JJ, Technical and clinical validation of a new immunoradiometric assay for human osteocalcin. Eur J Endocrinol 1996;135:231–7. ISSN 0804–4643
The measurement of circulating osteocalcin or bone GLA protein (BGP) constitutes a well established and non-invasive means for evaluating preferentially the bone formation rate, but most available commercial assays suffer from several technical constraints, notably a rapid degradation of BGP at room temperature or after thawing and the inability to measure subnormal values. We evaluated, from a technical and a clinical viewpoint, a newly available two-site sandwich immunoradiometric assay (IRMA) using standards of human origin and two different monoclonal antibodies. The theoretical and functional assay detection limit was 0.3 ng/ml. Concentrations of BGP progressively decreased when the serum was left at 4°C or at room temperature (mean apparent loss of 15% after 24 h). Two cycles of freezing–thawing only slightly reduced the BGP concentrations. The mean (± SD) BGP concentration was 19.6 ± 7.9 ng/ml in healthy subjects (NI, N = 61); the normal range was 8.1–35.6 ng/ml. There was a marked difference between pre- and postmenopausal women: 15.1 ±4.4 vs 22.3 ± 8.4 ng/ml, respectively (p<0.05). The mean BGP concentration in patients with tumor-induced hypercalcemia (N = 29) was not significantly different from NI, but nine patients (31%) had subnormal levels and five (17%) had elevated BGP levels. Concentrations of BGP were significantly increased in patients with hyperparathyroidism (N = 14) (45.1 ± 21.0 ng/ml) and significantly lower than NI in patients with hypoparathyroidism (N = 18) (7.3 ± 4.6 ng/ml). Concentrations of BGP were also measured by a classical radioimmunoassay using bovine standards and tracer; the correlations between both sets of measurements were significant in all groups, except in patients with hypoparathyroidism. In summary, this newly available IRMA for measuring circulating human BGP appears to be quite sensitive, reproducible and robust. It should be especially useful for investigating clinical conditions characterized by a low bone formation rate.
JJ Body, Institut Jules Bordet, Rue Héger-Bordet 1, 1000 Brussels, Belgium
This article has been cited by other articles:
|
|
 |
|
  Y. Vistoropsky, M. Keter, I. Malkin, S. Trofimov, E. Kobyliansky, and G. Livshits Contribution of the putative genetic factors and ANKH gene polymorphisms to variation of circulating calciotropic molecules, PTH and BGP Hum. Mol. Genet., May 15, 2007; 16(10): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Chung, C.-H. Ku, T.-Y. Chao, J.-C. Yu, M. M. Chen, and S.-H. Lee Tartrate-resistant Acid phosphatase 5b activity is a useful bone marker for monitoring bone metastases in breast cancer patients after treatment. Cancer Epidemiol. Biomarkers Prev., March 1, 2006; 15(3): 424 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Mancini, J.-C. Dumon, and J.-J. Body Efficacy and Safety of Ibandronate in the Treatment of Opioid-Resistant Bone Pain Associated With Metastatic Bone Disease: A Pilot Study J. Clin. Oncol., September 1, 2004; 22(17): 3587 - 3592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-M. Kakonen, J. Hellman, M. Karp, P. Laaksonen, K. J. Obrant, H. K. Vaananen, T. Lovgren, and K. Pettersson Development and Evaluation of Three Immunofluorometric Assays That Measure Different Forms of Osteocalcin in Serum Clin. Chem., March 1, 2000; 46(3): 332 - 337. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
