HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Robert, B. Articles by Duc-Goiran, P. Search for Related Content PubMed Articles by Robert, B. Articles by Duc-Goiran, P.

Expression of endothelin precursor genes in human trophoblast in culture

Robert B, Malassiné A, Bourgeois C, Mignot T-M, Cronier L, Ferré F, Duc-Goiran P. Expression of endothelin precursor genes in human trophoblast in culture. Eur J Endocrinol 1996;134:490–6. ISSN 0804–4643

We have shown previously the presence of immunoreactive endothelin in cultured trophoblastic cells from human term placenta as well as in the trophoblast-conditioned medium. To confirm whether or not the differentiated syncytiotrophoblast is a site for endothelin synthesis, we investigated, by reverse transcription and polymerase chain reaction, the expression of the three preproendothelin genes in 3-day cultured trophoblast. While no endothelin-2 precursor mRNA was detected, preproendothelin-1 mRNA was found to be expressed by the trophoblast. The endothelin-3 precursor gene was also expressed, but at low level and it was detected only after Southern blotting and oligonucleotide hybridization. The ability of trophoblast in culture to express the endothelin precursor genes supports the idea that, in human term placenta, villous syncytiotrophoblast that lines the intervillous space containing maternal blood acts as an endothelial layer.

B Robert, U.361 INSERM, Maternité Baudelocque, 123 Bld de Port-Royal, 75014 Paris, France

This article has been cited by other articles:

				C Niger, A Malassine, and L Cronier Calcium channels activated by endothelin-1 in human trophoblast J. Physiol., December 1, 2004; 561(2): 449 - 458. [Abstract] [Full Text] [PDF]

				M. V. St-Pierre, M. A. Serrano, R. I. R. Macias, U. Dubs, M. Hoechli, U. Lauper, P. J. Meier, and J. J. G. Marin Expression of members of the multidrug resistance protein family in human term placenta Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2000; 279(4): R1495 - R1503. [Abstract] [Full Text] [PDF]

				E. Jauniaux, T.-M. Mignot, R. Rebourcet, B. Robert, and F. Ferre Placental endothelin gene expression and endothelin concentration in fetal fluids of the first trimester gestational sac Mol. Hum. Reprod., August 1, 2000; 6(8): 758 - 762. [Abstract] [Full Text] [PDF]

				M. Napolitano, F. Miceli, A. Calce, A. Vacca, A. Gulino, R. Apa, and A. Lanzone Expression and Relationship between Endothelin-1 Messenger Ribonucleic Acid (mRNA) and Inducible/Endothelial Nitric Oxide Synthase mRNA Isoforms from Normal and Preeclamptic Placentas J. Clin. Endocrinol. Metab., June 1, 2000; 85(6): 2318 - 2323. [Abstract] [Full Text]

				C. Cudeville, F. Mondon, B. Robert, R. Rebourcet, T.-M. Mignot, C. Benassayag, and F. Ferré Evidence for Progesterone Receptors in the Human Fetoplacental Vascular Tree Biol Reprod, March 1, 2000; 62(3): 759 - 765. [Abstract] [Full Text]

				C. Bourgeois, B. Robert, R. Rebourcet, F. Mondon, T.-M. Mignot, P. Duc-Goiran, and F. Ferre Endothelin-1 and ETA Receptor Expression in Vascular Smooth Muscle Cells from Human Placenta: A New ETA Receptor Messenger Ribonucleic Acid Is Generated by Alternative Splicing of Exon 3 J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 3116 - 3123. [Abstract] [Full Text] [PDF]