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Stage-specific expression of c-myc messenger ribonucleic acid in porcine granulosa cells early in follicular growth

Sato A, Bo M, Maruo T, Yoshida S, Mochizuki M. Stage-specific expression of c-myc messenger ribonucleic acid in porcine granulosa cells early in follicular growth. Eur J Endocrinol 1994;131:319–22. ISSN 0804–4643

In the present study, the expression of c-myc mRNA in porcine granulosa cells harvested from varying follicle stages was analyzed using northern blot hybridization with a c-myc probe, and compared during follicular growth. Northern blot analysis revealed that porcine granulosa cell RNA obtained from small (1–2 mm) follicles contained an abundance of c-myc transcript of 2.4kb, the size of which was identical to the c-myc transcript obtained with human early placental RNA used as the control. In contrast to small-follicle granulosa cell RNA, total RNA preparations from either medium (3–5 mm)-follicle granulosa cells or large (6–11 mm)-follicle granulosa cells exhibited no detectable transcripts with the c-myc probe. These results suggest that expression of c-myc mRNA in porcine granulosa cells is limited only at the early stage of follicular growth. The stage-specific expression of c-myc mRNA in granulosa cells early in follicular growth may play a physiological role in the autonomous growth of immature granulosa cells in early folliculogenesis, seemingly independent of pituitary gonadotropic stimulation.

Takeshi Maruo, Department of Obstetrics and Gynecology, Kobe University School of Medicine, Chuo-ku, Kobe 650, Japan