HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Östenson, C.-G. Articles by Efendic, S. Search for Related Content PubMed Articles by Östenson, C.-G. Articles by Efendic, S.

Inhibition by rat diazepam-binding inhibitor/acyl-CoA-binding protein of glucose-induced insulin secretion in the rat

Östenson C-G, Ahrén B, Karlsson S, Knudsen J, Efendic S. Inhibition by rat diazepam-binding inhibitor/ acyl-CoA-binding protein of glucose-induced insulin secretion in the rat. Eur J Endocrinol 1994;131:201–4. ISSN 0804–4643

Diazepam-binding inhibitor (DBI) has been localized immunohistochemically in many organs. In porcine and rat pancreas, DBI is present in non-B-cells of the pancreatic islets. Porcine peptide also has been shown to suppress insulin secretion from rat pancreas in vitro. Recently, acyl-CoA-binding protein (ACBP) was isolated from rat liver and shown to be identical structurally to DBI isolated from rat brain. Using this rat DBI/ACBP, we have studied its effects on glucose-stimulated insulin secretion in the rat, both in vivo and in isolated pancreatic islets. Infusion iv of rDBI/ACBP (25 pmol/min) during glucose stimulation induced a moderate and transient reduction of plasma insulin levels. Moreover, rDBI/ACBP suppressed insulin release from batch-incubated isolated islets, stimulated by 16.7 mmol/l glucose, by 24% at 10 nmol/l (p < 0.05) and by 40% at 100 nmol/l (p < 0.01). The peptide (100 nmol/l) also inhibited the insulin response to glucose (16.7 mmol/l) from perifused rat islets by 31% (p < 0.05), mainly by affecting the acute-phase response. Finally, incubation of isolated islets in the presence of rDBI/ACBP antiserum (diluted 1:100 and 1:300) augmented the insulin response to 16.7 mmol/l glucose (p < 0.05 or even less). We conclude that rDBI/ACBP, administered iv or added to the incubation media, suppresses insulin secretion in the rat but that the effect is moderate despite the high concentration used. It is therefore unlikely that the peptide modulates islet hormone release, acting as a classical hormone via the circulation. However, the occurrence of DBI/ACBP in the islets and the enhancing effect by the rDBI/ACBP antibodies on glucose-stimulated insulin release suggest that the peptide is a local modulator of insulin secretion.

C-G Östenson, Department of Endocrinology, Karolinska Hospital, S-171 76 Stockholm, Sweden

This article has been cited by other articles:

				O. Lesouhaitier, M. K. Kodjo, F. Cartier, V. Contesse, L. Yon, C. Delarue, and H. Vaudry The Effect of the Endozepine Triakontatetraneuropeptide on Corticosteroid Secretion by the Frog Adrenal Gland Is Mediated by Activation of Adenylyl Cyclase and Calcium Influx through T-Type Calcium Channels Endocrinology, January 1, 2000; 141(1): 197 - 207. [Abstract] [Full Text] [PDF]

				J. V. Swinnen, P. Alen, W. Heyns, and G. Verhoeven Identification of Diazepam-binding Inhibitor/Acyl-CoA-binding Protein as a Sterol Regulatory Element-binding Protein-responsive Gene J. Biol. Chem., August 7, 1998; 273(32): 19938 - 19944. [Abstract] [Full Text] [PDF]

				C. K. Schjerling, R. Hummel, J. K. Hansen, C. Borsting, J. M. Mikkelsen, K. Kristiansen, and J. Knudsen Disruption of the Gene Encoding the Acyl-CoA-binding Protein (ACB1) Perturbs Acyl-CoA Metabolism in Saccharomyces cerevisiae J. Biol. Chem., September 13, 1996; 271(37): 22514 - 22521. [Abstract] [Full Text] [PDF]